ImmuHub®
ImmuHub®部分科研应用方向
ImmuHub®是由艾沐蒽开发的一套灵活度极高的TCR和BCR二代测序平台。可根据不同样本类型和课题的需求为客户提供个性化的免疫组测序。免疫组测序技术在肿瘤治疗、疫苗研发、生物标志物的发现、微小残留病检测、自身免疫性疾病的研究以及移植后监测等多个方面都具有重要价值。
部分免疫组测序相关文献
一、对治疗的反应监控、评估（Response to Therapeutics）：
- 监控联合使用免疫检查点抑制剂（PD-1和CTLA-4）对治疗爆发性心肌炎、黑色素瘤（Fulminant Myocarditis）效果。
Fulminant Myocarditis with Combination Immune Checkpoint Blockade.
November, 2016
TCR beta sequencing to determine clonal T-cell populations in melanoma patients undergoing immunotherapy. ICI 2016 | August, 2016
- 监测免疫检查点抑制剂CTLA-4治疗前列腺病（prostate cancer）、早期乳腺癌（breast cancer）的效果。
Clonal expansion of CD8 T cells in the systemic circulation precedes development of ipilimumab-induced toxicities. PNAS | October, 2016
Deep Sequencing of T-Cell Receptor DNA as a biomarker of clonally expanded TILs in breast cancer after immunotherapy. Cancer Immunology Research | September, 2016
- 评估PD-1抑制剂对免疫系统副作用反应。
PD-1 blockade induces responses by inhibiting adaptive immune resistance.
Nature | November, 2014
- 联合PD-L1和单抗对转移性肾瘤的治疗监控
Atezolizumab in combination with bevacizumab enhances antigen-specific T-cell migration in metastatic renal cell carcinoma. Nature Communications | August, 2016
- 评估使用DC疫苗和肿瘤浸润细胞回输方法对治疗恶性黑色素瘤的效果
A phase I clinical trial combining dendritic cell vaccination with adoptive T cell transfer in patients with stage IV melanoma Cancer Immunology. Immunotherapy | November, 2014
- 监测VGX-3100疫苗（DNA免疫治疗药物）用于宫颈上皮内瘤变（(HPV)16/18-positive CIN2/3），指导用药
Augmentation of cellular and humoral immune responses to HPV16 and HPV18 E6 and E7 antigens by VGX-3100. |September, 2016
- 对使用传统化疗方法治疗B细胞淋巴瘤效果的评价
The T-cell receptor repertoire influences the tumor microenvironment and is associated with survival in aggressive B-cell lymphoma Clinical Cancer. Research | September, 2016
- 监测使用AM0010（增强CD8+T细胞的存活率及其杀伤活性） 治疗胰腺癌或结直肠癌（pancreatic or colorectal cancer）的效果
Anti-tumor activity of PEGylated human IL-10 (AM0010) in patients with pancreatic or colorectal cancer. 2016 ASCO Annual Meeting | June, 2016
- 监控病毒感染后T细胞的动态反应，可指导设计有效疫苗
Dynamics of the Cytotoxic T Cell Response to a Model of Acute Viral Infection J Virol. | February, 2015
- 监控CD40抗体治疗转移性黑色素瘤的完全缓释进程
Immune activation and a 9-year ongoing complete remission following CD40 antibody therapy and metastasectomy in a patient with metastatic melanoma
Cancer Immunology Research | November, 2014
- 监控调节性T细胞治疗方法对抗宿主病的治疗效果
Clinical Grade Manufacturing of Human Alloantigen-Reactive Regulatory T Cells for Use in Transplantation. American Journal of Transplantation | September, 2013
二、疾病诊断（Disease Diagnosis）
- CD8 T细胞淋巴白血病（CD8+ T-large granular lymphocyte leukemia）诊断和疾病进展监控。
Deep sequencing of the T cell receptor repertoire in CD8+ T-large granular lymphocyte leukemia identifies signature landscapes. Blood | December, 2013
- 皮肤T细胞淋巴癌（Cutaneous T Cell Lymphoma）早期诊断和MRD检测。 TCR Sequencing Facilitates Diagnosis and Identifies Mature T Cells as the Cell of Origin in CTCL. Science Translational Medicine | October, 2015
Minimal Residual Disease Monitoring with High-Throughput Sequencing of T Cell Receptors in Cutaneous T Cell Lymphoma. Science Translational Medicine | December, 2013
- 从多肌炎到癌变的预测、检测。
Peripheral T-cell lymphoma emerging in a patient with aggressive polymyositis: molecular evidence for neoplastic transformation of an oligoclonal T-cell infiltrate. Acta Neuropathologica | October, 2013
- T细胞淋巴癌和白血病（T cell leukemias and lymphomas）的诊断以及治疗后监控。
Detecting and monitoring lymphoma with high-throughput sequencing. Oncotarget | April, 2011
三、免疫缺陷（Immunodeficiency）
- 无症状LIG4综合征免疫分型检测。
Ligase-4 Deficiency Causes Distinctive Immune Abnormalities in Asymptomatic Individuals. Journal of Clinical Immunology | May, 2016
- 普通变异性免疫缺陷病(common variable immunodeficiency，CVID)检测。Clonal and constricted T cell repertoire in Common Variable Immune Deficiency. Clinical Immunology | January, 2015
四、传染病（Infection Disease）
- HIV-1病人疾病的恶化监控。
IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection. JCI | June, 2016
- 肺结核疫苗（新药）评价。
Human and Murine Clonal CD8+ T Cell Expansions Arise During Tuberculosis Because of TCR Selection. PLOS Pathogens | May, 2015
五、 肿瘤（Solid Tumor）
- 通过对肿瘤润侵T细胞受体测序为肿瘤病人个性化照料提供依据。
Tumor-Associated Immune Parameters for Personalized Patient Care Science. Translational Medicine | December, 2013
- T细胞治疗效果监控。
High-content molecular profiling of T-cell therapy in oncology Molecular Therapy – Oncolytics | May, 2016
- 胶质瘤DC疫苗效果预测。
TCR Sequencing Can Identify and Track Glioma-Infiltrating T Cells after DC Vaccination. Cancer Immunology Research | March, 2016
六、自身免疫和炎症疾病（Autoimmunity/Inflammatory Disease）
- 先天性关节炎和类风湿性关节炎非侵入性（相对于关节腔液检查）检测。TCR repertoire sequencing identifies synovial Treg cell clonotypes in the bloodstream during active inflammation in human arthritis. Annals of Rheumatic Disease | June, 2016
- IgG4相关性疾病（IgG4-RD）的诊断。
Clonal expansion of CD4+ cytotoxic T lymphocytes in patients with IgG4-related disease. The Journal of Allergy and Clinical Immunology | March, 2016
七、移植免疫(Transplantation Immunity)
- 对移植术后环磷酰胺（PTCy）预防移植物抗宿主病(GVHD)的效果监控。
Origin and evolution of the T cell repertoire after posttransplantation cyclophosphamide. Journal of Clinical Investigation – Insight | May, 2016
- 脐带血造血干细胞移植后免疫重建的监控，评估干细胞移植后细胞免疫力重建程度，用于指导后天性或先天性免疫抑制患者的临床决策
Correlation of TCR diversity with immune reconstitution after cord blood transplant.
2012 American Society of Clinical Oncology Annual Meeting | May, 2012
TCR Repertoire Diversity Assessed with Immunosequencing is Associated with Patient Mortality Following Cord Blood Transplant. 2015 European Hematology Association Congress | June, 2015
Evolution and Clinical Implications of the T cell Repertoire Following Cord Blood Transplant. Biology of Blood and Marrow Transplantation | February, 2013
- 肾脏和骨髓移植（CKBMT）后免疫耐受的生物标记物检测。
Tracking donor-reactive T cells: Evidence for clonal deletion in tolerant kidney transplant patients. Science Translational Medicine | January, 2015
- 自身免疫/炎症性疾病
- 糖尿病-1型病人的BCR测序分析
Tissue distribution and clonal diversity of the T and B cell repertoire in type 1 diabetes
Howard R. Seay, Erik Yusko,Stephanie J. Rothweiler, Lin Zhang, et al.
JCI Insight. 2016;1(20):e88242.
https://insight.jci.org/articles/view/88242
- 系统性红斑狼疮病人用药后的BCR测序评估
Short-term assessment of BCR repertoires of SLE patients after high dose glucocorticoid therapy with high-throughput sequencing
Bin Shi, Jiang Yu, Long Ma, Qingqing Ma, Chunmei Liu,Suhong Sun, et al.
Shi, B., Yu, J., Ma, L. et al.
SpringerPlus 2016; 5: 75.
https://doi.org/10.1186/s40064-016-1709-4
- 多发性硬化症患者的BCR测序分析
Intrathecal BCR transcriptome in multiple sclerosis versus other neuroinflammation: Equally diverse and compartmentalized, but more mutated, biased and overlapping with the proteome
Jorunn N.Johansen, FrodeVartdala, CindyDesmarais, Astrid E.V.Tutturen, etal.
Clinical Immunology. 2015;160(2):211-225
http://www.sciencedirect.com/science/article/pii/S152166161500203X
- Wiskott-Aldrich氏症候群患者的BCR测序分析
Altered BCR and TLR signals promote enhanced positive selection of autoreactive transitional B cells in Wiskott-Aldrich syndrome
Nikita S. Kolhatkar, Archana Brahmandam, Christopher D. Thouvenel, etal.
Journal of Experimental Medicine. 2015;212(10):1663
http://jem.rupress.org/content/212/10/1663.full
- 传染性疾病
- 丙肝病毒（HCV）患者的BCR测序分析
Novel E2 Glycoprotein Tetramer Detects Hepatitis C Virus−Specific Memory B Cells
Maude Boisvert, Wanrui Zhang, Elizabeth J. Elrod, Nicole F. Bernard, etal.
Journal of Immunology. 2016;1600763
https://doi.org/10.4049/jimmunol.1600763
- 疾病诊断，血液学/肿瘤学
- 肺癌患者肿瘤中BCR测序分析
A high density of tertiary lymphoid structure B cells in lung tumors is associated with increased CD4+ T cell receptor repertoire clonality
Zhu W, Germain C, Liu Z1, Sebastian Y, Devi P, Knockaert S. etal.
OncoImmunology. 2015; 4(12)
https://doi.org/10.1080/2162402X.2015.1051922
- 白血病/淋巴瘤MRD检测
Detecting and monitoring lymphoma with high-throughput sequencing
Harlan Robins
Oncotarget. 2011; 2(4): 287–288.